Horticultural Characteristics of Transgenic Tobacco Expressing the rolC Gene from Agrobacterium rhizogenes
نویسندگان
چکیده
منابع مشابه
Assessment of salt tolerance in transgenic tobacco (Nicotiana tobacum L.) plants expressing the AUX gene
Transformation of plants using Agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. In this study, T-DNA from Ri plasmid in A. rhizogenes carrying pRi15834-PRT35S-GUS was introduced into tobacco leaf segments to initiate development of transformed hairy roots. Plant regeneration from transgenic roots used MS medium, and plants regenerated fro...
متن کاملResponses of Transgenic Tobacco (Nicotiana plambaginifolia) Over-Expressing P5CS Gene Underin vitroSalt Stress
Salinity is a major limiting factor for plant growth and development. To evaluate the impact of P5CS gene expression under in vitro salt stress condition, transgenic tobacco (Nicotiana plumbaginifolia) carrying P5CS gene and non-transgenic plants were treated with 0, 100, 150, 200 or 250 mM NaCl for 28 days. Proline content, lipid peroxidation and the activity of some antioxidant enzymes after ...
متن کاملCell-autonomous behavior of the rolC gene of Agrobacterium rhizogenes during leaf development: a visual assay for transposon excision in transgenic plants.
We describe a genetic switch based on the Ac transposable element of maize and the rolC gene of Agrobacterium rhizogenes, a dominant gene, which has pleiotropic effects on plant growth and morphology. Moreover, rolC gene expression under the control of the 35S cauliflower mosaic virus promoter decreases chlorophyll content in transgenic tobacco plants. Chlorophyll is a visible cell-autonomous m...
متن کاملassessment of salt tolerance in transgenic tobacco (nicotiana tobacum l.) plants expressing the aux gene
transformation of plants using agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. in this study, t-dna from ri plasmid in a. rhizogenes carrying pri15834-prt35s-gus was introduced into tobacco leaf segments to initiate development of transformed hairy roots. plant regeneration from transgenic roots used ms medium, and plants regenerated fro...
متن کاملgingseng (Panax ginseng) cells with the rolC gene from agrobacteria Agrobacterium rhizogenes can also cause the elevation of both the polyploidy and aneuploidy
The numbers of chromosomes and nucleoli in cultured cells of Vitis amurensis transformed with the rolB oncogene from A. rhizogenes have been studied. In general, the integration of the rolB gene in grape DNA mostly caused the elevation of the level of the chromosome variability, as well as higher numbers of nucleoli in the cultured cells. The possible influence of the observed chromosomal modif...
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ژورنال
عنوان ژورنال: Journal of the American Society for Horticultural Science
سال: 1994
ISSN: 0003-1062,2327-9788
DOI: 10.21273/jashs.119.5.1091